We are living today when, after almost two thousand years since the New Testament was completed, many genuine born-again Christians seem to have no idea what Biblical Separation is all about. Because of this, this subject should be studied and explained clearly for everyone who wishes to read about it. The author noted every Bible verse in the Old and New Testaments that dealt with this theme whether directly or by illustration. There are 1,896 verses in all. This important topic of Biblical Separation has been divided into six chapters: (1) Preliminary Considerations (2) Separation Regarding Things; (3) Separation Regarding Marriage; (4) Separation Regarding Unbelievers; (5) Separation Regarding Disorderly Believers; and (6) Summary And Conclusions. These chapters are followed by 9 pages of Scripture passages and index of subjects.